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Bruce M Applegate

Department of Food Sciences 

  • Associate Professor Food Science

B.A., Ph.D., University of Tennessee

Detection of viable foodborne pathogens using bateriophage; automated extraction of nucleic acids from various matrices; enumeration of microorganisms (i.e. pathogens and other organisms) using quantitative PCR; the use of bioreporters in bioelectronics; metabolic engineering; detection of problematic microorganisms in industrial environments; construction of recombinant bacterial strains to rapidly evaluate antimicrobial products; microbial ecology.

Awards & Honors

(2009) NASA Inventions and Contributions Board Award. NASA.

(2005) Seed for Success Award. Vice Provost for Research.

(2005) Seed for Success Award. Vice Provost for Research.


Applegate, B. M., Morgan, M., Perry, 0., & Kothapalli, A. Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface. U.S. Patent No. Filed Utility Patent # 12/549,500.. Washington, D.C.: U.S. Patent and Trademark Office.

Applegate, B. M. Bioluminescent biosensor device. U.S. Patent No. 6,544,729. Washington, D.C.: U.S. Patent and Trademark Office.

Selected Publications

Kim, S., Schuler, B., Terekhov, A., Auer, J., Mauer, L., & Perry, L. (2009). A bioluminescence-based assay for enumeration of lytic bacteriophage. Journal of Microbiological Methods, 79(1), 18-22.

Stratton, T., Garc, A., & Youngblood, J. (2009). Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency. Biomacromolecules, 10(5), 1173-1180.

Perry, L., SamMiguel, P., Minocha, U., Terekhov, A., Shroyer, M., Farris, L., Bright, N., . . . Reuhs, B. (2009). Sequence analysis of Escherichia coli O157:H7 bacteriophage FV10 and identification of a phage encoded immunity protein that modifies the O157 antigen. Fems Microbiology Letters, 292(2), 182-186.

Applegate, B. (2008). Chlorine Dioxide Oxidation of Dihydronicotinamide Adenine Dinucleotide (NADH) Inorganic Chemistry, 47, 2205-2211.

Habteselassie, M., Bischoff, M., Blume, E., Applegate, B., Reuhs, B., Brouder, S., & Turco, R. (2008). Environmental controls on the fate of Escherichia coli in soil. Water, Air and Soil Pollution, 190, 143-155.

Park, M., Banks, M., Applegate, B., & Webster, T. (2008). Influence of nanophase titania topography on bacterial attachment and metabolism. International Journal of Nanomedicine, 4(3), 8-Jan.

Applegate, B. (2008). Luminescence Techniques for the Detection of Bacterial Pathogens. In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems (213-230). Springer Science and Business Media.

Kim, S., Igboegwu, E., Terekhov, A., & Applegate, B. (2005). Bioluminescent Assay for Evaluating Bacteriophage Infectivity in a Food Model.

Hartano, L., Shroyer, M., Farris, L., Diefus-Dux, H., Turco, R., Reuhs, B., & Applegate, B. (2005). Development of an Escherichia coli O157:H7 Bioluminescent Reporter for Measuring Bioavailable Carbon.

Sedgley, C., Nagel, A., Hall, D., & Applegate, B. (2005). Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro. International Endodontics Journal, 38(2), 97-104.