Spring Semester 2018
For this week please read Exercise 7 in detail. Liquid cultures from white and blue colonies (see pages 37 and 38) have been prepared for you; you will extract plasmid DNA from these cultures (see pages 38 and 39) and then you will digest the extracted DNA with EcoRI and HindIII (see pages 39 and 40) in order to confirm that the restriction map of the fragment you cloned last week is consistent with published data.
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Lab 4 lecture.PDF Lab 6 lecture.PDF Lab 11 lecture.PDF
To view the steps of cloning (the summary of Labs 4 to 9), click on the icon below.