Fall Semester 2016
Last week you amplified a 1.3 kb DNA fragment from Arabidopsis TTG gene in order to clone it into a cloning vector. In this week you will electrophorese the amplified DNA to confirm that the target region was indeed amplified; then you will clone the amplified DNA into a TOPO vector, introduce the recombinant vector into E. coli competent cells, propagate the transformed E. coli cells, and culture the cells on agar plate to isolate the cloned fragment. Please read Chapter 6 in detail and pay more attention to Figures 6-4 and 6-5.
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To view lab lectures, please click on the icons below.
Lab 1 lecture.PDF Lab 2 lecture.PDF Lab 3 lecture.PDF Lab 4 lecture.PDFLab 5 lecture.PDFLab 6 lecture.PDFLab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDFLab 10 lecture.PDFLab 11 lecture.PDFLab 12 lecture.PDFLab 13 lecture.PDFLab 14 lecture.PDFLab 15 lecture.PDF
To view the Steps of Cloning (summary of Labs 4 to 9), please click on the icon below.
Steps of Cloning.PDF