Fall semester 2017
This week you will learn the technique of in vitro amplification of DNA. You will amplify a portion of the DNA, that you extracted last week, in order to clone it into a cloning vector. Please read Exercise 5 in detail and pay more attention to the Figure 5-3. Figure 5-4 (pages 26 to 28) shows the nucleotide sequence of the gene of interest. The region that you will amplify is located between nucleotide pairs 2573 and 3872 (see page 27).
This week is the quiz week: Quiz #1 covers Exercises 1 to 4.
To view the lectures, click on the icons below.
Lab 1 lecture.PDFLab 2 lecture.PDFLab 3 lecture.PDFLab 4 lecture.PDFLab 5 lecture.PDFLab 6 lecture.PDFLab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDF Lab 10 lecture.PDFLab 11 lecture.PDFLab 12 lecture. PDFLab 13 lecture.PDFLab 14 lecture.PDFLab 15 lecture.PDF
To view the steps of cloning (the summary of Labs 4 to 9), click on the icon below.
Steps of Cloning.PDF