Spring Semester 2018 

                                                 

 Lab 7

For this week please read Exercise 7 in detail.  Liquid cultures from white and blue colonies (see pages 37 and 38) have been prepared for you; you will extract plasmid DNA from these cultures (see pages 38 and 39) and then you will digest the extracted DNA with EcoRI and HindIII (see pages 39 and 40) in order to confirm that the restriction map of the fragment you cloned last week is consistent with published data.

 

 

 

 

 

To view the course syllabus, please click on the icon below.

          Syllabus.PDF   Course Syllabus.PDF

 

 

 

 To view lectures, please click on the icons below.                                                                                                                                                     

 

Lab 1 lecture.PDFLab 2 lecture.PDFLab 3 lecture .PDF    Lab 4 lecture.PDFLab 4 lecture.PDF    Lab 5 lecture.PDFLab 6 lecture.PDFLab 6 lecture.PDF      Lab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDF Lab 10 lecture.PDFLab 11 lecture.PDFLab 11 lecture.PDF        Lab 12 lecture.PDF Lab 13 lecture.PDFLab 14 lecture.PDF  Lab 15 lecture.PDF

 

 To view the steps of cloning (the summary of Labs 4 to 9), click on the icon below.

Steps of Cloning.PDF