Spring Semester 2017
In this week you will electrophorese last week's digested plasmids (see Figure 7-2 on page 39, and Figure 8-2 on page 42) in order to confirm that the size and the physical map of the fragment you cloned are consistent with published data. Then you will denature the digested fragments and will do the Southern. A transfer set up for Southern blotting is shown in Figures 8-1 and 8-3.
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Lab 1 lecture.PDF Lab 2 lecture.PDF Lab 3 lecture.PDF Lab 4 lecture.PDFLab 5 lecture.PDFLab 6 lecture.PDFLab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDFLab 10 lecture.PDFLab 11 lecture.PDFLab 12 lecture.PDFLab 13 lecture.PDFLab 14 lecture.PDFLab 15 lecture.PDF
To view the Steps of Cloning (summary of Labs 4 to 9), please click on the icon below.
Steps of Cloning.PDF